Découvrez les derniers papiers de recherche publiés au sein de la Biofonderie de Paris
Restez informé des dernières avancées et des projets en cours au Biofoundry DNA et Microbe. Notre recherche est à la pointe de l’ingénierie microbienne et de la biologie synthétique, faisant progresser les innovations en matière d’assemblage d’ADN, de phénotypage microbien et d’ingénierie biologique durable. Des découvertes pionnières aux études collaboratives, nos mises à jour présentent le travail de pointe qui façonne l’avenir de la biotechnologie.
Découvrez ci-dessous les récentes réalisations et développements de nos chercheurs :
Stéphane Lemaire, David Turek, Dave Landsman, Marthe Colotte, Tom de Greef
Résumé
Pas de résumé disponible.
Engineering Photosynthetic Microorganisms in Biofoundries: Challenges and Opportunities
Damien Douchi, Mathieu Husser, Lucile Jomat, Christophe Marchand, Pierre Crozet, Stéphane Lemaire
Résumé
Photosynthetic microorganisms can convert sunlight and CO₂ directly into biomass and bioproducts. Yet, most biofoundries still optimize heterotrophic chassis reliant on agricultural sugars, limiting impact on global decarbonization. This review argues that sustainable manufacturing requires integrating microalgae and cyanobacteria into Design–Build–Test–Learn pipelines and shifting from biomass conversion to light- and CO₂-driven production. We highlight advances in genetic and cellular engineering in model photosynthetic microbes including modular cloning, genome editing, and organelle engineering that enable pathway design for lipids, isoprenoids, and proteins. We discuss phototroph-specific bottlenecks for automation and standardization, including slower growth, variable transgene expression, chlorophyll autofluorescence, and the need for controlled illumination and gas exchange with linked data pipelines. Finally, we examine cultivation and scale-up constraints, emphasizing co-optimization of strain traits, reactor design, and downstream processing to improve techno-economic and environmental performance. Photosynthetic biofoundries are therefore both necessary and increasingly feasible for a low-carbon bioeconomy.
Antoine Van de Vloet, Lucas Prost-Boxoen, Quinten Bafort, Yunn Thet Paing, Griet Casteleyn, Lucile Jomat, Stéphane Lemaire, Olivier De Clerck, Yves Van de Peer
Résumé
Whole-genome duplications, widely observed in plant lineages, have significant evolutionary and ecological impacts. Yet, our current understanding of the direct implications of ploidy shifts on short- and long-term plant evolution remains fragmentary, necessitating further investigations across multiple ploidy levels. Chlamydomonas reinhardtii is a valuable model organism with profound potential to study the impact of ploidy increase on the longer term in a laboratory environment. This is partly due to the ability to increase the ploidy level. We developed a strategy to engineer ploidy in C. reinhardtii using noninterfering, antibiotic, selectable markers. This approach allows us to induce higher ploidy levels in C. reinhardtii and is applicable to field isolates, which expands beyond specific auxotroph laboratory strains and broadens the genetic diversity of parental haploid strains that can be crossed. We implement flow cytometry for precise measurement of the genome size of strains of different ploidy. We demonstrate the creation of diploids, triploids, and tetraploids by engineering North American field isolates, broadening the application of synthetic biology principles in C. reinhardtii. However, our newly formed triploids and tetraploids show signs of rapid aneuploidization. Our study greatly facilitates the application of C. reinhardtii to study polyploidy, in both fundamental and applied settings.
Félix de Carpentier, Stéphane Lemaire, Antoine Danon
Résumé
The unicellular green alga Chlamydomonas reinhardtii is a valuable model system to study a wide spectrum of scientific fields, including responses to environmental conditions. Most studies are performed under optimal growth conditions or under mild stress. However, when environmental conditions become harsher, the behavior of this unicellular alga is less well known. In this review we will show that despite being a unicellular organism, Chlamydomonas can survive very severe environmental conditions. To do so, and depending on the intensity of the stress, the strategies used by Chlamydomonas can range from acclimation to the formation of multicellular structures, or involve programmed cell death.
Konstantinos Vavitsas, Pierre Crozet, Marcos Hamborg Vinde, Fiona Davies, Stéphane Lemaire, Claudia E Vickers
Résumé
Photosynthetic microorganisms offer novel characteristics as synthetic biology chassis, and the toolbox of components and techniques for cyanobacteria and algae is rapidly increasing.